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Here is an example of how a quick and simple cleaning can restore column performance. In HPLC, peak splitting in an established method that was previously performing well, is most often attributed to column contamination at the column head. The method in question is a fast screening method with a 25mM pH 2.6 phosphate buffer and methanol. The "After" chromatogram is typical of the method (i.e. fully resolved peaks were never needed in this method and resolution was sacrificed for speed). Cleaning was done by reversing the column and running 100% isopropanol at 0.25ml/min for one hour.

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