Navigation

Vial fill effecting reproducibility was mentioned in an Agilent document called something like "Maximizing Reproducibility in GC/MS Analysis." In this paper one of the suggestions was consistent vial fill volume with a suggested fill of 1mL. Can vial fill level have an impact on reproducibility? To find out I ran five replicates at 0.5mL, 1.0mL and 1.5mL. All samples were transferred using a Rainin EDP pipette. The method is a rapid ethanol analysis with acetonitrile as the internal standard and acetone as the solvent. The working ethanol concentration was 0.6% with a 100:1 split ratio and a injection volume of 0.1uL. A 5uL syringe was in the 7683 ALS. As you can see the 1mL fill was indeed the most reproducible.