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Welcome to DreamingSpirals. Why Dreaming Spirals? Partly because I published a zine with that name (ca. 1994) and partly because it's all about spirals.More about the author can be found here.
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Minimizing the delay volume on the Agilent 1100.
posted at 07:54PM on Wednesday November 29
Agilent recently published information on decreasing the delay volume on the 1100. I like to save time whenever possible so I decided to give this a try. The method in question is the same one that I described here.
The method was modified with an injector program to switch the valve to bypass at one minute into the run. This is before the gradient starts so switching back to main-pass before during the next injection will not effect the retention times or the second sample. As you can see the elution time of the last peak decreased significantly.
This decrease and setting the autosampler to pre-fetch the net vial saves about 50 seconds per sample. This can add up fast when you have 20 or more samples to run.
This decrease and setting the autosampler to pre-fetch the net vial saves about 50 seconds per sample. This can add up fast when you have 20 or more samples to run.
Comments
milkshake wrote:
12/02/06 23:15:36
George Perham wrote:
It would be interesting to see if the 1200 is configured to switch to bypass automatically based on injection volume.
12/03/06 10:42:34
Cheers!